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A conservative region of the mercuric reductase gene (merA) as a molecular marker of bacterial mercury resistance BJM
Sotero-Martins,Adriana; Jesus,Michele Silva de; Lacerda,Michele; Moreira,Josino Costa; Filgueiras,Ana Luzia Lauria; Barrocas,Paulo Rubens Guimarães.
The most common bacterial mercury resistance mechanism is based on the reduction of Hg(II) to Hg0, which is dependent of the mercuric reductase enzyme (MerA) activity. The use of a 431 bp fragment of a conservative region of the mercuric reductase (merA) gene was applied as a molecular marker of this mechanism, allowing the identification of mercury resistant bacterial strains.
Tipo: Info:eu-repo/semantics/article Palavras-chave: MerA gene; Mercury resistant; Bacteria; Molecular marker.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200020
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Transmission of Campylobacter coli in chicken embryos BJM
Rossi,Daise Aparecida; Fonseca,Belchiolina Beatriz; Melo,Roberta Torres de; Felipe,Gutembergue da Silva; Silva,Paulo Lourenço da; Mendonça,Eliane Pereira; Filgueiras,Ana Luzia Lauria; Beletti,Marcelo Emilio.
Campylobacter coli is an important species involved in human cases of enteritis, and chickens are carriers of the pathogen mainly in developing country. The current study aimed to evaluate the transmission of C. coli and its pathogenic effects in chicken embryos. Breeder hens were inoculated intra-esophageally with C. coli isolated from chickens, and their eggs and embryos were analyzed for the presence of bacteria using real-time PCR and plate culture. The viability of embryos was verified. In parallel, SPF eggs were inoculated with C. coli in the air sac; after incubation, the embryos were submitted to the same analysis as the embryos from breeder hens. In embryos and fertile eggs from breeder hens, the bacterium was only identified by molecular methods;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Campylobacter coli; Viability; Transmission; Breeder hens.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200014
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